解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Micro-needle arrays increase skin permeability by forming channels through the outer physical barrier, without stimulating pain receptors populating the underlying dermis. It was postulated that micro-needle arrays could facilitate transfer of DNA to human skin epidermis for cutaneous gene therapy applications. Platin...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860500383705
更新日期:2005-08-01 00:00:00
abstract::The human papillomavirus type-16 (HPV-16) E7 protein is considered a major viral oncoprotein involved in cervical cancer (CxCa) and a potential candidate for the development of a vaccine against this neoplasia. Here, two lactic acid bacteria (the model one Lactococcus lactis and a probiotic one Lactobacillus plantarum...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860400024219
更新日期:2005-02-01 00:00:00
abstract::Phage display technology has been widely used for developing tumor-targeting agents. Most of the efforts were directed towards identifying phage-displayed ligands against cancer-relevant purified targets and cancer cell lines. Whole cell screening procedures typically use a relatively large sample size and are not ide...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860400020464
更新日期:2005-01-01 00:00:00
abstract::An oral vaccine formulation comprised of starch microparticles with conjugated antigens is being developed. In this report we have examined the uptake of such microparticles by the intestinal mucosa and examined whether the conjugated antigen can influence the uptake. Two model antigens were used: recombinant cholera ...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/1061186042000223662
更新日期:2004-06-01 00:00:00
abstract::The purpose of this research is to develop ligand-targeted plasmid based gene delivery systems for gene transfer to tumor endothelium. Cell adhesion assays were used to test the peptide inhibition of human endothelial cell adsorption to vitronectin-treated tissue culture plates. A series of RGD containing peptides wer...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860410001724468
更新日期:2004-05-01 00:00:00
abstract::Most therapeutic agents are delivered orally. Consequently, the major classes of therapeutically useful chemicals are partially lipophilic, small molecular weight compounds. They have reasonable permeability coefficient values across cell membranes, including those of intestinal epithelia and vascular endothelia. In c...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
doi:10.1080/10611860410001693715
更新日期:2004-02-01 00:00:00
abstract::An in vivo study has been performed in order to determine the influence of amphotericin B (AMB) molecular organization on the toxicity and activity of this drug in the treatment of experimental visceral leishmaniasis. Three formulations with similar composition but different drug molecular self-association in aqueous ...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860400006596
更新日期:2004-01-01 00:00:00
abstract::Recently, considerable interest has been focussed on the use of biodegradable polymers for specialized applications such as controlled release of drug formulations; meanwhile, microsphere drug delivery systems using various kinds of biodegradable polymers have been studied extensively during the past two decades. In t...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860400010630
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:The polycationic nanopeptide RKKRRQRRR is a fragment of HIV-Tat protein known to be a transfection enhancer. Such factors control the transfection efficiency of plasmid DNA complexed with tat peptide. However, their actions are poorly understood. METHODS AND RESULTS:Several cell lines and primary cells were...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/106118604200041403
更新日期:2004-01-01 00:00:00
abstract::The present work describes the potential for using lectin-conjugated gliadin nanoparticles as a means of locating and anchoring a drug delivery system on the carbohydrate receptors of Helicobacter pylori (H. pylori). Gliadin nanoparticles (GNP) bearing acetohydroxamic acid (AHA) were prepared by a desolvation method. ...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860310001647771
更新日期:2003-08-01 00:00:00
abstract::Biphenylylacetic acid (BPAA) was linked to the free hydroxyl group of 2,6-di-O-methyl-beta-Cyclodextrin (DM-beta-CyD) through an ester linkage to obtain the site specific release of the drug to the colon. The conjugate at 1:1 mole ratio was separated from the reaction mixture by semipreparative reverse-phase HPLC and ...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860310001615965
更新日期:2003-05-01 00:00:00
abstract::Improved delivery of macromolecular drugs to solid tumor is known as the enhanced permeability and retention (EPR) effect of macromolecular drugs and lipids. We report here that a prostaglandin I2 (PGI2) analogue induces enhancement of tumor-selective drug delivery, while it decreases tumor blood flow, in a rat tumor ...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/1061186031000086072
更新日期:2003-01-01 00:00:00
abstract::Archaeal ether glycerolipid vesicles (archaeosomes) efficiently deliver exogenous antigen for induction of humoral and cell-mediated immunity. Because induction of CD8 cytotoxic T cells is critical for protective vaccination against tumors, we compared the ability of various archaeosome lipid compositions to evoke a s...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860410001670044
更新日期:2003-01-01 00:00:00
abstract::Chemotherapy is still a major challenge for in vivo drug targeting to macrophages. Toxicity remains the major obstacle for the most potent drugs already known in the therapy of leishmaniasis. Thus, new drugs and new delivery systems are sought. By using different vesicular delivery modes e.g. liposomes, niosomes, micr...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/106118021000072681
更新日期:2002-12-01 00:00:00
abstract::HEK cells were transfected with the GFP gene using various vectors: naked DNA, lipofectamine, and both PLGA and P(FASA) plasmid-loaded nanospheres. All methods were assessed alone and with the use of chloroquine, a lysosomal enzyme inhibitor. Transfection efficiencies were determined and compared at various times post...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/1061186021000038373
更新日期:2002-09-01 00:00:00
abstract::Based on the organotropic characteristics of bile acids towards the liver and the intestine, two novel compounds of the Bamet family, containing at least one bile acid moiety bound to platinum(II), have been synthesized and their cytostatic effect compared to their ability to become accumulated in tumor cells of hepat...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/1061186021000001841
更新日期:2002-08-01 00:00:00
abstract::Recent studies have shown that drugs that are normally unable to cross the blood-brain barrier (BBB) following intravenous injection can be transported across this barrier by binding to poly(butyl cyanoacrylate) nanoparticles and coating with polysorbate 80. However, the mechanism of this transport so far was not know...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860290031877
更新日期:2002-06-01 00:00:00
abstract::In vitro transfection efficiency of a plasmid DNA for rat gastric mucosal (RGM)-1 cells was enhanced by ultrasound (US) irradiation. Ethylenediamine was introduced to the carboxyl groups of gelatin to prepare a cationized gelatin as the vector of plasmid DNA encoding luciferase. An electrophoresis experiment revealed ...
journal_title:Journal of drug targeting
pub_type: 杂志文章,收录出版
doi:10.1080/10611860290022624
更新日期:2002-05-01 00:00:00
abstract::Gene augmentation has been the paradigm in the majority of gene therapy protocols but in recent years the potential of repairing the mutated gene in situ by targeted gene correction has become a reality. In fact, targeted gene repair has many advantages over conventional replacement strategies, notably the possibility...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
doi:10.1080/10611860290016739
更新日期:2002-03-01 00:00:00
abstract::Targeting of anti-tumor drugs to the urinary bladder for the treatment of bladder carcinoma may be useful, since these agents generally have a low degree of urinary excretion and are highly toxic elsewhere in the body. The anti-tumor drug doxorubicin was coupled to the low-molecular weight protein lysozyme via the aci...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860290007568
更新日期:2002-02-01 00:00:00
abstract::Germ-free (GF) animals lack a colonic microflora like that seen in conventional (CV) animals. Bacterial presence plays a role in the development of glycoproteins in the gastrointestinal (GI) tract; the absence of a microbiota has been seen to suppress the production of certain glycoproteins and glycolipids. Binding pa...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611860108997920
更新日期:2001-04-01 00:00:00
abstract::The transport of 125I-radiolabelled latex nanoparticles across the nasal mucosa of rats was studied using a range of particle sizes and surface coatings. Translocation of the particles into the blood stream was examined by means of monitoring the radiolabel associated with the particles. Particles were detected in the...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611860108997935
更新日期:2001-01-01 00:00:00
abstract::Gene transfer offers a potential way to correct local and systemic protein deficiency disorders by using genes as drugs, so called gene therapeutics. Salivary glands present an interesting target site for gene therapeutic applications. Herein, we review proofs of concept achieved for salivary glands with in vivo anima...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
doi:10.3109/10611860108998782
更新日期:2001-01-01 00:00:00
abstract:UNLABELLED:Polyethyleneimine (PEI) can be used as a DNA delivery mechanism in cell culture and in vivo. Cells can be transfected by using surface-bound PEI, as well as by PEI/DNA microparticles. In the present experiments we extended these observations by preparing microspheres with covalently attached PEI. Blends of p...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611860108995629
更新日期:2001-01-01 00:00:00
abstract::Interactions between phosphatidylcholine (PC) or phosphatidylserine (PS) liposomes and human umbilical vein endothelial cells (HUVEC) or human promyelocytic leukemia cells (HL60) were investigated. Pyramine encapsulating or rhodamine incorporating small unilamellar liposomes with mean diameters around 80 nm (demonstra...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611860008997910
更新日期:2000-01-01 00:00:00
abstract::Several experimental approaches have been tested for suicide gene delivery into tumor cells, including viral and non-viral vectors. In this study, we compared the efficiency of Herpes Simplex Virus type 1 thymidine kinase gene (HSV-tk) delivery by retrovirus-producing cells and DNA/liposome complexes for the treatment...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611860008996854
更新日期:2000-01-01 00:00:00
abstract::The biodistribution of orally and intravenously administered archaeosomes in mice was compared to that of archaeosomes containing either coenzyme Q10 (archaeosome-CoQ10), polyethylene glycol (archaeosome-PEG), or PEG plus CoQ10 (archaeosome-PEG-CoQ10). The archaeosome formulations were prepared by a reverse-phase evap...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869909085521
更新日期:2000-01-01 00:00:00
abstract::Poly(D,L-lactic-co-glycolic) acid (PLGA) microspheres containing plasmid DNA encoding the firefly luciferase gene were prepared using the water-in-oil-in-water (w/o/w) double emulsion and solvent evaporation method. In this study, we investigated the effects of three process parameters on DNA microencapsulation: (1) e...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869909085514
更新日期:1999-12-01 00:00:00
abstract::Gelatin microspheres (ms) and gelatin/BSA (bovine serum albumin) or gelatin/alginate ms were prepared by encapsulating fluorescein isothiocyanate (FITC) labeled dextran or interferon alpha (IFN-alpha). Ms were obtained by an emulsion-solvent-extraction method. Gelatin and gelatin/BSA ms were obtained by treating water...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869908996851
更新日期:1999-01-01 00:00:00
abstract::Supramolecules since ages have been characterised as self-associating systems emanating the properties directly related to the mode of molecular association. Similar to supramolecules, liquid crystals may also be considered as an associated system. The liquid crystals are defined as the state of matter with the charac...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869908996839
更新日期:1999-01-01 00:00:00
abstract::Local tissue toxicity, systemic toxicity and platinum pharmacokinetics were evaluated in 6 normal healthy beagle dogs injected subcutaneously with two formulations of a polylactide biodegradable polymer (Atrigel) system containing cisplatin. Dogs were injected 4 times at 30 day intervals at platinum dosages of 70, 105...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869808997866
更新日期:1998-01-01 00:00:00
abstract::The aim of the present study was to evaluate the transfer of N-acetyl-p-aminobenzoic acid (AcPABA) across the rat term placenta and the rat small intestine and to compare it with that of its parent drug p-aminobenzoic acid (PABA). Umbilical perfusion of the rat term placenta was used to determine the materno-fetal tra...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869808995875
更新日期:1998-01-01 00:00:00
abstract::Highly lipophilic antitumor agent, palmitoyl rhizoxin (RS-1541), was incorporated into stable lipid emulsions about 100-1000nm in mean diameter consisting of triglyceride ODO and surfactant HCO-60. The pharmacokinetics of RS-1541 were studied after i.v. injection in mice, rats, rabbits, and dogs. Dog showed characteri...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869808997875
更新日期:1998-01-01 00:00:00
abstract::The high-molecular-weight soluble aggregates of Fab fragments of murine antibodies against cardiac myosin were prepared as a potential long-circulating and low immunogenic pharmaceutical carriers by conjugation of thiolated Fab and Fab modified with succinimidyl 3-(2-pyridyldithio)propionate. The clearance time and bi...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869808997880
更新日期:1998-01-01 00:00:00
abstract::This study was designed to exploit the ability of Peyer's patch M cells to recognize antigen-antibody complexes in the targeted delivery of a model antigen for the induction of mucosal immunity. Sensitized liposomes consisted of an entrapped model antigen, ovalbumin (OVA), and coated with unrelated antigen-antibody co...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869708995854
更新日期:1997-01-01 00:00:00
abstract::I125 radiolabelled HSA (HSA-I125) was utilised as a probe to quantify protein adsorption onto polystyrene (PS) and Poloxamine 908 coated PS (PS-908) particles. Upon ageing of the HSA-I125 a dramatic increase in the amount of protein adsorbed onto the two particle systems was observed. This phenomenon was not due to la...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869708995849
更新日期:1997-01-01 00:00:00
abstract::Liposomes have been prepared from dipalmitoylphosphatidylcholine (DPPC), cholesterol (Chol) and dimethyldioctadecylammonium bromide (DDAB). The cationic vesicles adsorb to biofilms of the skin-associated bacteria Staphylococcus epidermidis, which have a negative charge. Encapsulation of the antibacterial drug vancomyc...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869609015975
更新日期:1996-01-01 00:00:00
abstract::A general mathematical model involving partition coefficient, molecular weight and hydrogen bonding is used to correlate the structures and permeability of various organic compounds through the toad urinary bladder and human red blood cell (RBC) membranes. Log Per (permeability) is correlated with log Po/w (partition ...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869609046268
更新日期:1996-01-01 00:00:00
abstract::We have developed a new method to evaluate the transfer of macromolecular drugs to intrapulmonary lymph nodes in rats after intrapulmonary administration. By using this method, we observed that the transfer of fluorescein isothiocyanate dextrans (FDs) to the intrapulmonary lymph nodes was markedly higher than that to ...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869509015955
更新日期:1995-01-01 00:00:00
abstract::A simple method for the separation of polyalkylcyanoacrylate nanoparticles was developed using polyisohexylcyanoacrylate (PIHCA) as a model. Fluorescein isothiocyanate dextran 70 was used to label the nanoparticles. Ultracentrifugation onto a performed sucrose gradient allowed the easy elimination of the dextran and o...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869509059216
更新日期:1995-01-01 00:00:00